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When the sperm count is very low ,then a single sperm is injected in to the egg. Even if sperm are absent in ejaculate, then sperm can be extracted from testis ( Testicular Sperm extraction). it needs a micromanipulator with which an egg is held by suction and with a sharp needle a single sperm is injected and the fertilization is achieved. It is a boon for the couple where the routine IVF is not successful because of failed fertilization by conventional IVF or where the sperm count is very low or sperms are nonmotile.

The stimulation protocol for the ICSI is same as that of conventional IVF. Eggs are surgically removed as in IVF . In normal circumstances the eggs are surrounded by cluster of cells called cumulus corona cells ,all of which must removed before the sperm injection takes place. Removal of the cells gives an insight of the eggs to embryologist ,to assess the maturity of the egg . Sperms are collected from the male partner , either by masturbation or they are surgically retrieved from testis.

Denuded eggs are placed in a dish under 200 or 400 magnification with a inverted microscope fitted with the micromanipulator .We have Nikon Narishige combinations of ultimate quality . The eggs is around 100 micron in width and is held by a glass holding pipette with a diameter of 40 to 50 micron usually on the left side . Injection needle is heving a diameter of five to six micron.

idealfertility.com

1st Floor, Deshbandhu Complex, Naudra Bridge,
Jabalpur, MP INDIA –
482001
Telephone + 91- 761 -2627711
Mobile 91 - 0 – 9826166952




ICSI-Quality Infertility Treatments

In order for pregnancy to occur, an egg has to be released from the ovary and unite with a sperm. Normally this union, called fertilization, occurs within the fallopian tube, which joins the uterus (womb) to the ovary. However, in IVF the union occurs in a laboratory after eggs and sperm have been collected. Embryos are then transferred to the uterus to continue growth.

The retrieval procedure to obtain the eggs is performed trans-vaginally using a hollow needle guided by the ultrasound image (this is completely comfortable under adequate sedation and local anesthesia). Eggs are gently removed from the ovaries using the needle. This is called "follicular aspiration." Its timing is crucial because the egg will not develop properly if it is collected too early; if too late, the egg also may develop poorly or may have already been released from the ovary and lost.

The eggs are immediately identified by our embryologists in our special IVF laboratory. They are placed with the sperm in incubators to allow fertilization to take place. The eggs are examined carefully at intervals to ensure that fertilization and cell division have taken place; the fertilized eggs are now called embryos.

Embryos are usually placed in the wife's uterus 2 or 3 days after egg retrieval. A speculum is inserted into the vagina to expose the neck of the womb (cervix). The embryos are suspended in a tiny drop of fluid and then very gently introduced through a catheter into the womb, often under ultrasound guidance. The transfer is followed by a resting period, blood tests and possibly ultrasound examinations to verify if pregnancy has been established.

www.infertilityindia.com

LILAVATI ASSISTED CONCEPTION UNIT,
2ND FLOOR, LILAVATI HOSPITAL,
BANDRA WEST, MUMBAI - 400 050.
2- 643 8280 EXTN 2- 622 1111 (Extn 2245)




Malpani Infertility Clinic

However there is a dark side to IVF as well. All the media hype about IVF has created a lot of pressure on infertile couples. Most family members and friends naively think that IVF represents a sure shot success method, and cannot understand why infertile couples balk at going through IVF treatment. Moreover, since the media only highlights the success stories, patients themselves often have unrealistic expectations from IVF treatment. Unscrupulous clinics which quote highly exaggerated and misleading pregnancy rates simply add to the confusion.

Media coverage about infertility has also given birth to a new medical condition I call “pre-infertility”. Preinfertile couples are usually young couples with no medical problems, who start to panic because they hear their biological clocks ticking. Even though they have no medical problems, and would have conceived in their own bedroom if they had given themselves enough time, they often rush headlong into seeking medical assistance . Unfortunately, many doctors are more than happy to provide this even though it is not needed, because such patients boost the clinic’s success rates and give the doctor an undeserved reputation of being a good fertility specialist !

Because the treatment is so expensive, many doctors feel IVF is a lucrative business proposition, as a result of which many IVF clinics start simply to make money. They then pressurise infertile couples ( who could get pregnant with simpler treatment options) to go in for IVF, leading to unnecessary overtreatment. Since these are often young couples with minimal medical problems, their pregnancy rates with IVF are high, thus boosting the clinic’s success rate, and their reputation as well.

www.drmalpani.com

Jamuna Sagar,
Shahid Bhagat Singh Road,
Colaba, Bombay 400 005.
Tel: 91-22-22151065, 91-22-22151066
Fax (India): 91-22-2215 0223
 
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